Prof. Amparo Acker-Palmer
|Institute of Cell Biology and Neuroscience
Cluster of Excellence Macromolecular Complexes (CEF)
60438 Frankfurt am Main
The main purpose of our research is to study neuronal and vascular networks with focus on cell-cell communication via the ephrin/Eph system and their crosstalk with other angiogenic and neurotrophic factors. A growing body of evidence is emerging for common molecular mechanisms shared by nerves and blood vessels to grow, pattern and navigate. Eph receptors and ephrin ligands were the first example of axon guidance cues involved in the development of the vascular system. Moreover, over the last two decades, developmental biology has focused on the elucidation of the signaling pathways that shape the embryo. Now, it has been appreciated that such programs often govern analogous mechanisms in adult tissues. This is also very well represented by the EphB/ephrinB system. Therefore, our studies are not limited to embryogenesis but also extend to plasticity phenomena in the adult.
To study the influence of the Eph/ephrin system in the nervous and vascular systems we employ different cellular models including rat and mice primary cortical and hippocampal neurons, endothelial and endothelial precursor cells. As an animal model we use the mouse, including different transgenic as well as genetically modified mouse lines. We use a broad range of biochemical and cell biology methods, such as in vitro kinase assays, immunoprecipitation, Immunofluorescence and various migration, repulsion/adhesion and proliferation assays. We also use innovative techniques like time lapse imaging for the study of cell adhesion/repulsion induced by Eph and ephrin signaling. Moreover, my laboratory has successfully applied the TAP technology in the mammalian system, both in primary cells and in transgenic mice.
Pfennig S, Foss F, Bissen D, Harde E, Treeck JC, Segarra M, Acker-Palmer A. (2017). GRIP1 Binds to ApoER2 and EphrinB2 to Induce Activity-Dependent AMPA Receptor Insertion at the Synapse. Cell Rep, 3;21(1):84-96.